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500 iu il2  (Miltenyi Biotec)
99
Miltenyi Biotec 500 iu il2
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
500 Iu Il2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/500 iu il2/product/Miltenyi Biotec
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500 iu il2 - by Bioz Stars, 2026-03
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texmacs gmp complete medium with il-2 supplement (200 iu/ml) media bag  (Terumo BCT)
90
Terumo BCT texmacs gmp complete medium with il-2 supplement (200 iu/ml) media bag
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
Texmacs Gmp Complete Medium With Il 2 Supplement (200 Iu/Ml) Media Bag, supplied by Terumo BCT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/texmacs gmp complete medium with il-2 supplement (200 iu/ml) media bag/product/Terumo BCT
Average 90 stars, based on 1 article reviews
texmacs gmp complete medium with il-2 supplement (200 iu/ml) media bag - by Bioz Stars, 2026-03
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il-2 (20 iu/ml; novartis)  (Novartis)
90
Novartis il-2 (20 iu/ml; novartis)
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
Il 2 (20 Iu/Ml; Novartis), supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il-2 (20 iu/ml; novartis)/product/Novartis
Average 90 stars, based on 1 article reviews
il-2 (20 iu/ml; novartis) - by Bioz Stars, 2026-03
90/100 stars
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il-2 500 iu/ml proleukin  (Novartis)
90
Novartis il-2 500 iu/ml proleukin
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
Il 2 500 Iu/Ml Proleukin, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il-2 500 iu/ml proleukin/product/Novartis
Average 90 stars, based on 1 article reviews
il-2 500 iu/ml proleukin - by Bioz Stars, 2026-03
90/100 stars
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proleukin 6000 iu/ml recombinant human (rh) il-2  (Prometheus Laboratories)
90
Prometheus Laboratories proleukin 6000 iu/ml recombinant human (rh) il-2
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
Proleukin 6000 Iu/Ml Recombinant Human (Rh) Il 2, supplied by Prometheus Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proleukin 6000 iu/ml recombinant human (rh) il-2/product/Prometheus Laboratories
Average 90 stars, based on 1 article reviews
proleukin 6000 iu/ml recombinant human (rh) il-2 - by Bioz Stars, 2026-03
90/100 stars
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100,000 iu il-2  (Novartis)
90
Novartis 100,000 iu il-2
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
100,000 Iu Il 2, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/100,000 iu il-2/product/Novartis
Average 90 stars, based on 1 article reviews
100,000 iu il-2 - by Bioz Stars, 2026-03
90/100 stars
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recombinant human il- 2 600,000 iu  (Clinigen Inc)
90
Clinigen Inc recombinant human il- 2 600,000 iu
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
Recombinant Human Il 2 600,000 Iu, supplied by Clinigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human il- 2 600,000 iu/product/Clinigen Inc
Average 90 stars, based on 1 article reviews
recombinant human il- 2 600,000 iu - by Bioz Stars, 2026-03
90/100 stars
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recombinant human il-2 600,000 iu  (Clinigen Inc)
90
Clinigen Inc recombinant human il-2 600,000 iu
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
Recombinant Human Il 2 600,000 Iu, supplied by Clinigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human il-2 600,000 iu/product/Clinigen Inc
Average 90 stars, based on 1 article reviews
recombinant human il-2 600,000 iu - by Bioz Stars, 2026-03
90/100 stars
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500 iu/ml il-2  (PeproTech)
90
PeproTech 500 iu/ml il-2
FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of <t>IL2,</t> and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.
500 Iu/Ml Il 2, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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500 iu/ml il-2 - by Bioz Stars, 2026-03
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FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of IL2, and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.

Journal: Cancer Immunology Research

Article Title: Complement Factor H Is an ICOS Ligand Modulating Tregs in the Glioma Microenvironment

doi: 10.1158/2326-6066.CIR-23-1092

Figure Lengend Snippet: FH enhances the immunosuppressive function of Tregs. A, FH increases the immunosuppressive effect of Tregs. Tregs were incubated in medium only or supplemented with 150 μg/mL FH. CD4 + T cells were isolated from the same donor and cocultured with Tregs in the presence of CD2/CD3/CD28 MACSiBeads and 3 H-thymidine. Secretion of IL10 ( B and D ) and TGFβ ( C ) by Tregs incubated in medium only, 150 μg/mL α1-AT, FH, or dFH. Cells were stimulated with anti-CD3 and anti-CD28 microbeads in the presence of IL2, and secreted cytokines were measured by ELISA after 48 hours ( B ) or 72 hours ( C ). D, Frequency of IL10-producing Tregs, either unstimulated, or stimulated for 16 hours with TransAct, TransAct + FH, TransAct + dFH, or TransAct + SEA, relative to ICOS expression, was assessed with IL10 catch. Data are means ± SD of n = 7 ( A ), n = 6 ( B ), n = 4 ( C ) independent experiments; representative plots of n = 5 ( D ) independent experiments. Statistical tests: two-way ANOVA ( A ) and one-way ANOVA ( B and C ) with Tukey’s multiple comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. AF, Alexa Fluor; CPM, count per minute; CTRL, control; ns, nonsignificant; SEA, staphylococcal enterotoxin A; Tresp, responder CD4 + T cells.

Article Snippet: T cells were cultured in TexMACS medium (#130-097-196; Miltenyi Biotech) with 20 IU (CD4 + , CD8 + , and naïve T cells) or 500 IU IL2 (Tregs; #130-097-743; Miltenyi Biotech).

Techniques: Incubation, Isolation, Enzyme-linked Immunosorbent Assay, Expressing, Comparison, Control